Free laboratory template

Peptide COA Review Worksheet

Fifteen fields for connecting a peptide certificate to the sample, lot, method, chromatogram, reported results, limitations, and laboratory disposition.

Version August 29, 2026 · Print, CSV, and JSON

Use one record for one certificate and lot.

Review every field against the original report and received package. Record the evidence, not just a pass or fail. If information is missing or ambiguous, mark it for review rather than inferring a favorable result.

Download CSV Download JSON

This worksheet does not validate a certificate or establish identity, purity, quantity, sterility, safety, or fitness. It organizes the evidence used in a laboratory's own review.

Printable review record

Certificate and lot review

Material / product
Lot / batch
Report ID
Reviewer / date

Peptide certificate of analysis review fields
Review field	Question and evidence	Status / notes
01 · DocumentReport identifier	Does the record have a unique report or certificate identifier? Record: Report number, revision, and source URL or file name.	□ Confirmed □ Review □ Not reported □ N/A
02 · DocumentTesting laboratory	Is the laboratory clearly identified with a verifiable contact or public record? Record: Laboratory name, location or contact, and verification source.	□ Confirmed □ Review □ Not reported □ N/A
03 · DocumentDates	Are sample receipt, analysis, and report dates stated and internally consistent? Record: Receipt date, analysis date, issue date, and revision date.	□ Confirmed □ Review □ Not reported □ N/A
04 · TraceabilitySubmitted sample	Does the submitted material name and stated strength match the package and purchase record? Record: Product name, sequence or blend where applicable, strength, and format.	□ Confirmed □ Review □ Not reported □ N/A
05 · TraceabilityLot or batch	Does one unique lot or batch connect the report, physical package, and receiving record? Record: Lot or batch from each source and any discrepancy.	□ Confirmed □ Review □ Not reported □ N/A
06 · TraceabilityPackage linkage	Can the tested sample be connected to the received package without relying on an unsupported assumption? Record: Label image, receiving record, chain-of-custody record, or unresolved gap.	□ Confirmed □ Review □ Not reported □ N/A
07 · Analytical resultsIdentity	Which method supports identity, and what result and acceptance criterion were reported? Record: Method, observed result, expected result, specification, and conclusion.	□ Confirmed □ Review □ Not reported □ N/A
08 · Analytical resultsChromatographic purity	Is the purity value tied to a named chromatographic method and calculation? Record: Method, detector, area percentage, specification, and conclusion.	□ Confirmed □ Review □ Not reported □ N/A
09 · Analytical resultsChromatogram	Is the underlying trace available with axes, peak labels, integration, and sample identity? Record: Trace location, main retention time, included peaks, and any integration concern.	□ Confirmed □ Review □ Not reported □ N/A
10 · Analytical resultsQuantity or content	Was peptide amount measured separately from chromatographic area percentage? Record: Method, result, units, uncertainty where reported, specification, and conclusion.	□ Confirmed □ Review □ Not reported □ N/A
11 · Analytical resultsAdditional attributes	Which additional attributes were actually tested rather than implied? Record: Named test, method, result, units, and specification for each attribute.	□ Confirmed □ Review □ Not reported □ N/A
12 · Method reviewMethod context	Does the report provide enough method, reference, calibration, and sample-preparation context for the claim? Record: Method identifier, reference material, calibration, preparation, detector, and limitations.	□ Confirmed □ Review □ Not reported □ N/A
13 · Method reviewIndependent verification	Can the original record be verified directly with the issuing laboratory or public report system? Record: Verification URL, access code, confirmation date, and reviewer.	□ Confirmed □ Review □ Not reported □ N/A
14 · DecisionUntested attributes	Are sterility, endotoxin, safety, biological activity, stability, and fitness left unclaimed unless tested? Record: Explicit limitations and any unsupported claim requiring resolution.	□ Confirmed □ Review □ Not reported □ N/A
15 · DecisionReview disposition	Is the acceptance, rejection, quarantine, or information-request decision documented? Record: Disposition, rationale, reviewer, review date, and follow-up owner.	□ Confirmed □ Review □ Not reported □ N/A

Why the fields are separate

Identity, purity, and quantity require distinct evidence.

A peer-reviewed peptide reference-standard study describes using multiple analytical techniques and quantitative assignments to establish different attributes. The worksheet keeps identity, chromatographic purity, quantity, and additional tests separate so one result is not silently reused as proof of another.

The peptide purity guide explains why HPLC area percentage is not automatically peptide content by mass, while the HPLC vs LC-MS guide separates chromatographic and mass evidence.

Method selectivity

A clean-looking peak still needs method context.

Closely related components can coelute. Research on two-dimensional LC-MS assessment of pharmaceutical peptide peak purity illustrates why column, gradient, detector, and orthogonal evidence matter when a decision depends on resolving similar species.

For that reason, the worksheet asks for the chromatogram, integration, method identity, detector, reference, and limitations—not only the reported percentage.

Sources

Analytical references and worked source record.

COA worksheet FAQ

Preserve evidence and unresolved questions.

What is a peptide COA review worksheet?

It is a structured record for checking certificate identity, lot linkage, analytical methods, results, specifications, limitations, and the laboratory's review decision. It helps keep evidence and unresolved questions connected to the same sample.

Does completing this worksheet validate a peptide COA?

No. The worksheet organizes the review. It cannot authenticate a document, repair missing traceability, establish an untested attribute, or replace direct verification with the issuing laboratory and the receiving laboratory's qualification process.

Should HPLC purity and peptide quantity be recorded separately?

Yes. HPLC area percentage and quantitative peptide content answer different questions. Record the method, result, units, specification, and conclusion for each reported attribute rather than copying one percentage into both fields.

What status values does the downloadable worksheet use?

The JSON template defines confirmed, needs review, not reported, and not applicable. A laboratory can adapt those values to its controlled vocabulary while preserving the original evidence and review notes.

Can the worksheet be printed or imported into a spreadsheet?

Yes. The page is print-formatted, and the same 15-field template is available as CSV for spreadsheets and JSON for laboratory systems or custom workflows.

Research Use Only

Notice